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| Submitter's Name |
Maite De Maria |
| Session Name |
Poster Session - Water Quality - Water Management |
| Poster Number |
44 |
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| Author(s) |
Maite De Maria, Center of Environmental and human toxicology (Presenting Author) |
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Kevin Kroll,
Center of Environmental and human toxicology, Department of Physiological Science, University of Florida |
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Viet Dang, Center of Environmental and human toxicology, Department of Physiological Science, University of Florida |
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Mike Walsh, Aquatic Animal Health Program, University of Florida |
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Nancy Densolow, Center of Environmental and human toxicology, Department of Physiological Science, University of Florida |
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Nephrotoxic effects of glyphosate and Rodeo ® through water exposure on largemouth bass |
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Glyphosate is a component of herbicides with the largest use in the world and Florida is not an exception. It has been used extensively as a ripener for sugarcane and to control lake vegetation. Farmers chronically exposed to glyphosate have shown unusual kidney disease. In addition, after ingestion of large quantities, glyphosate produces nephrotoxicity among another organ failures. In the environment, fish can be exposed to runoff from agricultural areas or it can be sprayed directly into water bodies. Long-term exposure experiments with animal models, like fish, have shown reproductive impairments and kidney disease associated with glyphosate or its commercial version. Kidney injury can be related to the production of reactive oxygen species and it is the organ that may excrete glyphosate components. Our objective is to assess kidney damage and molecular changes in largemouth bass after chronic exposure to glyphosate or Rodeo, the commercial product. We exposed male individuals to 0.5 and 10mg/L concentrations of glyphosate and Rodeo® (chemically equivalent concentration) in water for 28 days. Our lowest concentration was similar to the EPA limit for drinking water. Glyphosate or its breakdown product (AMPA) will be determined in blood with mass spectrometry. We analyze changes in gene expression through RNA sequencing on trunk kidney and perform histopathology in this organ and others. We are going to correlate tissue damage with altered gene expression that will be validated by qPCR. Our genes of interest are kidney injury molecule (KIM-1), beta-2-microglobulin, neutrophil gelatinase-associated lipocalin (NGAL), corroborated with a housekeeping gene. Our research will contribute to identifying molecular markers for kidney damage as a consequence of exposure to glyphosate or Rodeo® in fish that can be extended to other species exposed through their environment. |
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