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| Submitter's Name |
Reyna Colli-Dula |
| Session Name |
Posters - Nutrient dynamics and enrichment impacts in aquatic ecosystems 2 |
| Category |
Nutrient dynamics and enrichment impacts in aquatic ecosystems |
| Poster Number |
45 |
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| Author(s) |
Reyna Cristina Colli-Dula, University of Florida (Presenting Author) |
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Alvine Mehinto,
University of Florida |
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Kevin J. Kroll, University of Floria |
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David Barber, University of Florida |
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Nancy Denslow, University of Florida |
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Understanding of Benzene and Trichloroethylene Effects in Largemouth Bass Using Microarrays |
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Benzene and trichloroethylene (TCE), known environmental pollutants, have been detected in aquatic systems causing adverse effects in fish. This is due to high usage and release of both industrial discharges and releases during oil extraction and transport as well as to high mobility of these compounds in the atmosphere. One way to explore mechanisms of toxicity of these compounds is through quantification of gene expression in fish. We used microarrays to investigate changes in gene expression caused by exposure to benzene and TCE in largemouth bass. Twelve fish were injected in each group for vehicle control as well as for each treatment (using a single dose of benzene or TCE 10 mg/kg b/w). After injection, the fish were sacrificed at 48h. Livers and gonads were obtained and analyzed for gene expression changes using microarrays. The hypothesis was that concentrations used for benzene and TCE would produce changes in sensitive genes. Resulting ANOVA analysis in the liver revealed 1272 and 1116 genes were significantly affected (p |
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